Tg mpeg1:mcherry

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August undefined, 2024

WebFirst, by performing L-plastin (Lcp1) immunohistochemistry and using transgenic Tg(mpeg1.1:GFP) or Tg(mpeg1.1:mCherry) fish, we analyzed the distribution of … Web12 May 2024 · To assess the behaviour of each macrophage subgroup, we imaged Tg (Mpeg1:mCherry) embryos over the course of one hour (acquisition every minute; Video 1 ). A colour-coded process was used to...

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Web7 Jan 2024 · Mpeg1.1 expression does not discriminate a subset of phagocytic B cells in zebrafish. (A) Distribution of fluorescence in cell suspension from the IPEX of adult … Web27 Jan 2011 · Several mpeg1-driven, tol2-flanked transgene constructs were built for recapitulating mpeg1 expression in live animals: Tg(mpeg1:EGFP); Tg(mpeg1:mCherry); … simpson timber post base

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WebA: Three still images from a confocal timelapse microscopy video showing the gradually increased expression of tnfa by macrophages after infection with E. coli via the duct of Couvier at 3 DPF, in the Tg(mpeg1:mCherry-F) ump2 [Reference Nguyen-Chi, Phan and Gonzalez 57] fishline, with macrophages expressing red fluorescent mCherry, crossed … Web10 Jun 2024 · A similar 10 mM short treatment regimen using Tg (mpx:KalTA4;UAS:nfsB-mCherry) larvae resulted in 4.9% ablation ( Fig. 1J and Supplementary Data ). In summary, here we present the Tg (lyz:YFP-NTR2.0) transgenic line as a new tool for fast and effective ablation of neutrophils in zebrafish. This line can achieve complete loss of neutrophils ... WebTg (mpeg1:mCherryCAAX) Type engineered_plasmid Regulatory Regions mpeg1.1 ( 1) Coding Sequences mCherry ( 1) [Em λ] [Ex λ] 610 (red) 587 (red) Fpbase:mCherry … simpson timothy

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Webmpeg1.1. Acts upstream of or within defense response to bacterium. Predicted to be located in cytoplasmic vesicle membrane. Is expressed in several structures, including blood … Web4 Aug 2024 · Next, we analyzed double transgenic cd45:CFP‐NTR;runx1:mCherry embryos at 48 and 72 hpf by flow cytometry, and triple transgenic cd45:CFP‐NTR;runx1:mCherry;mpeg1:GFP embryos at 60 hpf by live imaging (Appendix Fig S3A–C). Our data clearly show that CFP and mCherry never overlap, meaning that the 7.6 … razor pro x scooter wheelsWeb10 Aug 2024 · macrophages from the early stage of regeneration, Tg(mpeg1:mCherry-F) were injected with L-clodronate (or L-clo) or L-PBS (control) at 48 hpf and fin were … razor purple pot thrower

"WebTo further evaluate immunotoxicity of down and feather samples, we performed ZFET with macrophage-EGFP zebrafish, Tg(mpeg1: ... To create mCherry protein expressing E. coli, we transfected a pFCcGi plasmid (Addgene, Cambridge, MA, USA) to the DH5α bacteria with a standard protocol. We selected and established stable mCherry expressing cells ... " - Tg mpeg1:mcherry

Tg mpeg1:mcherry

ZFIN Feature: ihb21Tg - Zebrafish Information Network

Web10 Aug 2024 · mpeg1.1 + lymphoid-like cells are found in the spleen, blood, and caudal fin of adult Tg ( mpeg1.1:mCherry ); TgBAC ( csf1ra:GFP) zebrafish. ( A – C) Confocal imaging in the spleen (A), circulation (B), and caudal fin (C). Arrowheads identify mpeg1.1+ cells. (A and C) Whole-mount imaging in fixed tissues. WebVentricles of wildtype Tg ( mpeg1:mCherry) were collected into PBS containing 10 mM HEPES, 30 mM taurine, and 5.5 mM glucose and cells dissociated by the addition of 0.25% trypsin, 12.5 M CaCl 2, and 5 mg/mL Collagenase II …

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Web12 May 2024 · To assess the behaviour of each macrophage subgroup, we imaged Tg(Mpeg1:mCherry) embryos over the course of one hour (acquisition every minute; … Web(D–G) Confocal micrographs of transverse sections from 3 dpi MTZ+ Tg(mpeg1:mCherry; rpe65a:nfsB-eGFP) larval eyes. Cyan arrowheads point to MΦs/µglia (magenta) overlapping with RPE debris (green) in central–dorsal (D and E) and central–ventral (F and G) regions. Dorsal is up; anterior is left. (Scale bars, 20 μm.)

WebEggs obtained from Tg(mpeg1:mCherry) fish were transfected with DNA plasmids by microinjection into the yolk syncytial later that is formed at 3-4 hpf. The caudal vein tissue of the transfected embryos at 3 dpf was imaged every minute for 30 min. (Movie: Yuya Hayashi) Featured article Web30 Jul 2024 · Histological staining of isolated wt1b:eGFP +;mpeg1:mCherry + and mpeg1:mCherry + cells from cryoinjured hearts revealed a typical myeloid cell shape with large cytoplasmic areas and an irregular nuclear shape. Double-positive cells were slightly larger than mpeg1:mCherry + cells (Figures 1J and 1K), suggesting that wt1b:eGFP …

Web10 Feb 2024 · In brief, 4 dpf Tg(mpeg1:mCherry) larvae were subject to needle-stab injury and the injured region was dissected at 1, 2 and 3 dpi and tissue dissociated into a single … Webated a reporter line Tg(mpeg1:loxP-DsRedx-loxP-GFP), in which macrophages (Ellett et al., 2011) were DsRedx+ under normal conditions but switched to GFP+ upon Cre/loxP-medi-ated recombination. The reporter line was outcrossed with Tg(hsp70:mCherry-T2a-CreERT2) ﬁsh to generate double trans-genic line, in which IR-LEGO-CreER-loxP labeling ...

Web35 rows · Transgenic Construct Tg (mpeg1:mCherry) ID ZDB-TGCONSTRCT-120117-2 …

Web25 Feb 2024 · Transgenic Tg(mpeg1:mCherry) zebrafish embryos stably express mCherry (magenta) within all macrophages. b,c) Whole embryo (10× magnification) and tissue level (40× magnification) views of srLNP biodistribution and eGFP expression within the embryonic zebrafish at 1.5 hpi. At this timepoint, srLNPs were mainly associated with … simpson timber strap over sill platesWebThe same phenomenon was also found in Tg(coro1a:DsRed;mpeg1:GFP) double transgenic lines [37] ( Figure S2A). Through fluorescence co-localization analysis of DsRed + and … simpson timberlok screwsWebFigure Legend Snippet: ( A ) Maximum projection of a microglia (green) making contact with myelin sheaths (magenta) in a 4 dpf Tg(mpeg1:EGFP; sox10:mRFP) zebrafish spinal cord. ( B-F ) Microglia scanning behavior along myelin sheaths in the spinal cord was analyzed by confocal time-lapse imaging of 3-4 dpf Tg(mpeg1:EGFP; mbp:mCherry-CAAX) or … razor purchaseWeb20 Jun 2024 · We first assessed macrophage heterogeneity and recruitment dynamics following larval cardiac injury. We crossed the zebrafish pan-macrophage reporter line Tg(mpeg1:GFP) with Tg(csf1ra:gal4;UAS:mCherry-NfsB) (shortened here to csf1ra: mCherry) (Figure S1 A, related to Figure 1).Csf1ra (colony stimulated factor 1 receptor) is … razor push mower - 159ccWebTg (mpeg1:mCherryCAAX) ( 1) Type engineered_plasmid Regulatory Regions mpeg1.1 ( 1) Coding Sequences CAAX ( 1) mCherry ( 1) [Em λ] [Ex λ] 610 (red) 587 (red) … simpson timber to timber joist hangerWebSynonyms Tg2 (mpeg1:mCherry)ihb21 Affected Genomic Region This feature is representative of one or more unknown insertion sites. Construct Tg2 (mpeg1:mCherry) ( … razor purchase onlineWeb17 Jul 2024 · NADPH oxidase inhibitor Apocynin increases susceptibility to notochord infection. (A-B) E. coli- GFP were injected in the notochord of 2 dpf tg (mpx: GFP) embryos in DMSO or Apocynin treatment conditions. (A) At 1 dpi, superoxide production was visualised using DHE (red), neutrophils and E. coli were detected using GFP. razor put each item on a new row bootstrap