Shythdf3
WebJun 28, 2024 · Scale bar, 10 μm (n=3 independent experiments); c. Quantification of YTHDF3 protein expression by immuno-staining in shScram and shYTHDF3 cells (Scram, sh1, and sh2). WebDec 6, 2024 · Meanwhile, two short hairpin RNAs (shYTHDF3–1 and shYTHDF3–2) were transfected into Huh7 and SNU449 cells to construct YTHDF3-stably-knocked down cell …
Shythdf3
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WebFigure Legend Snippet: a , b mCherry-GFP-LC3 was transfected into shNS and shYTHDF3 MEFs, and autophagosome (yellow) and autolysosome (red) formation was examined. Scale bar, 20 μm. c , d Endogenous ULK1, ATG13, ATG14, and DFCP1 puncta in shNS and shYTHDF3 MEFs were immunostained after nutrient deficiency, visualized with confocal … Web5-GGACGTTCCCAATAGCCAACT-3; shYthdf3, 5-GCACCTAAACCAACTTCTTGG-3; shLacZ (con-trol),5-GCATAAACCCGCCACTCATCT-3.The lentiviral overexpression vector pLVX- IRES-eGFP was made from pLVX-IRES-Hyg by replacing HygR with eGFP. The lentiviral particles were generated using a second generation packaging system. In brief, HEK293T
WebMDA-MB231Br cells were transduced with shControl and shYTHDF3 lentivirus and selected by puromycin. Growth protocol: MDA-MB-231Br cells were grown in DMEM medium with 10% FBS and 1% penicillin-streptomycin. Cells were grown in a 5% CO2 cell culture incubator at 37℃. Extracted molecule: total RNA: Extraction protocol WebGAPDH is used as a loading control. d Immunoblot analyses of YTHDF3 restored (shYTHDF3 + YTHDF3) and control (shYTHDF3 + Con) MEFs following nutrient starvation …
WebMay 1, 2024 · Animals and generation of Ythdf1 cKO mice. For generation of Ythdf1 conditional knockout (cKO) mice, exon 4 of mouse Ythdf1 gene was targeted with the consideration that exon 4 encodes the YTD domain. A loxP site and an FRT-flanked neomycin resistance gene (Neo r) coupled with a loxP site were inserted in intron 3 and … WebJan 1, 2024 · N6-methyladenosine (m6A) constitutes one of the most abundant internal RNA modifications and is critical for RNA metabolism and function. It has been previously reported that viral RNA contains internal m6A modifications; however, only recently the function of m6A modification in viral RNAs has been elucidated during infections of HIV, …
WebThe objectives are to identify expression profiles that are specific to shYTHDF3 breast cancer cell to identify new the signaling pathway of YTHDF3 for brain metastasis of …
WebMar 10, 2024 · e, f qRT-PCR and immunoblot analysis of the indicated mRNA and protein in HTR8/SVneo transfected with shYTHDF3 for 72 h. g, i RNA lifetime for IGF1R in … diaper covers for girls dressesWebJul 27, 2024 · h Immunoblot analyses of FOXO3 rescued MEFs (shYTHDF3 + FOXO3) and control MEFs (shYTHDF3 + Con) following nutrient starvation for the indicated time … citibank na tickercitibank n.a. tokyo branchWebEV71 genomic RNA contains m 6 A modifications. (A) UHPLC-MS/MS. EV71 RNA was harvested from large-scale culture and subjected to UHPLC-MS/MS analysis.The percentage of m 6 A among A residues is presented on the y axis. (B and C) MeRIP-Northern blotting. RNAs from virus infected Vero cells (B) or from in vitro T7 transcription (C) were … citibank national association翻译Web1 day ago · However, xenografted U373MG (Uppsala) tumors expressing either shYTHDF3-targeting shRNAs were significantly more sensitive to PP242 as compared to tumors transduced with vector only or control scrambled non-targeting shRNA. We also determined m 6 A cyclin D1 and c-myc IRES RNA and protein levels in harvested tumors. citibank n.a. new york ny u.s.aWebDec 9, 2024 · Background METTL3 is an RNA methyltransferase that mediates m6A modification and is implicated in mRNA biogenesis, decay, and translation. However, the biomechanism through which METTL3 regulates MALAT1-miR-1914-3p-YAP axis activity to induce NSCLC drug resistance and metastasis is not very clear. Methods The expression … citibank n.a. wolfsberg questionnaireWebFigure Legend Snippet: a , b mCherry-GFP-LC3 was transfected into shNS and shYTHDF3 MEFs, and autophagosome (yellow) and autolysosome (red) formation was examined. Scale bar, 20 μm. c , d Endogenous ULK1, ATG13, ATG14, and DFCP1 puncta in shNS and shYTHDF3 MEFs were immunostained after nutrient deficiency, visualized with confocal … diaper cover shorts