Ribofecttmcp
Webb5 dec. 2024 · According to the instructions of RiboFECTTMCP transfection reagent (Guangzhou RiboBio Co., Ltd., Guangzhou, Guangdong, China), 100 ng JAG1‐wt or JAG1‐mut was co‐transfected with miR‐335‐5p NC or miR‐335‐5p mimic in a respective manner into 293 T cells and cultured in the 24‐well plate for 48 h. Three replicates were … Webband incubation with 50 μL RiboFECTTMCP buffer and 5 μL trans‐ fection reagents, …
Ribofecttmcp
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Webb13 mars 2024 · 转染方法 以riboFECT? CP Reagent 转染siRNA于24孔板，转染浓度为50nM为例，其他规格容器转染请参考表2。. 1. 接种细胞 a. 贴壁细胞：转染前一天，接种1×105细胞至含有适量完全培养基的24孔板培养孔中，使转染时的细胞密度能够达到50~80%。. 注：1）不同细胞生长速度不 ... WebbConclusions. IL-13 can increase the proliferation and migration of airway smooth muscle cells by promoting the expression of H19 and Orai1 and decreasing the expression of miR-93-5p. H19 aggregates airway inflammation and airway remodeling in murine models of asthma possibly by inhibition of the expression of Orai1.
WebbRESEARCH ARTICLE Resveratrol reduces the apoptosis induced by cigarette smoke extract by upregulating MFN2 Chao Song, Bailing Luo*, Li Gong Respiratory Department, Xiangya Hospital, Central South University, Changsha, Hunan Province,China * … Webb25 sep. 2024 · CCK-8 and Western blot were used to detect cell viability and apoptotic proteins in PC12 cells exposed to lead acetate. A, Detection of cell viability by CCK-8 assay.
Webb8 jan. 2024 · The transfection system of miR-124-5p was the mixture of 1× riboFECTTMCP Buffer, 100 ng/μl riboFECTTMCP Regent, and miR-185-5p mimic (80 nM), and the mixture was added drop-wise to the appropriate wells, respectively. Then, the medium with miRNA transfection was changed immediately before OGD. Webb本发明属于生物医药技术领域，特别涉及线粒体自噬抑制剂在制备提高肝癌细胞对索拉非尼的敏感性的药物中的应用。背景技术原发性肝细胞肝癌(hcc)是全球范围内发病率及死亡率最高的恶性肿瘤之一，2024年全球新发hcc病例达60万，其中有近一半发生在中国，是关乎我国人群健康的重大社会医学问题 ...
WebbA complete of 200?mol/L clear vector plasmid simply Duloxetine distributor because bad control (NC) or LINC01305, 100?ng plasmid (TNXB\wt or TNXB\mut), after incubation and mixture with 50?L RiboFECTTMCP buffer and 5?L transfection reagents, co\transfection with SiHa cells were performed relative to the guidelines of RiboFECTTMCP transfection …
WebbLong RNA Transfection. Cell transfection is one of the important methods for foreign genes entering into eukaryotic cells. Those foreign DNAs or RNAs move into cells by the ancillary packaging of transfection reagents, play its role to further study its cell functions and corresponding influences. mRNA transfection does not need to enter into ... hawkwind albums youtubeWebb20 apr. 2024 · According to the manufacturer’s protocol of transfection reagent, the … hawkwind albums ratedWebb细胞转染按照riboFECTTM CP 转染试剂盒说明书进行操作。实验设置3个组：阴性对照(miR-negative control, miR-NC)组，加入阴性对照mimic及riboFectTMCP 试剂；模拟物(miR-mimic)组，加入miR-200a mimics及riboFectTMCP 试剂；抑制剂(miR-inhibitor)组，加入miR-200a 抑制剂及riboFectTMCP 试剂。 hawkwind 25 years on box setWebb20 apr. 2024 · According to the manufacturer’s protocol of transfection reagent, the miRNAs was diluted with riboFECTTM CP buffer at 37°C for 10 min. The diluent was mixed with riboFECTTM CP Reagent and incubated for 10 min at 37°C. Then, the riboFECTTMCP-miRNA mixture was added to the cells together with 1.8 ml DMEM and incubated at 37°C … hawkwind and fire 下载Webb10 sep. 2024 · The treatment group was given two types of MSCs (1 × 10 6) in 100 μL of … hawkwind and fire mp3WebbTransfections were performed with the riboFECTTMCP Reagent (Ruibo, Guangzhon, China) according to the manufacturer's protocol. Cells were seeded in a 6-well plate at 1×10 5 /well, transfected with siRNA (target sequence: GCACCAAGAAGGTTCATGT, synthesized by Ruibo, Guangzhou, China), and cultured for 24 hours. hawkwind and fire百度网盘Webb9 juli 2024 · Next, 12 μl of riboFECTTM CP Reagent (RiboBio) was added and mixed by gentle pipetting, followed by incubation at room temperature for 0–15 minutes. Finally, riboFECTTMCP Transfection Complex was added to the appropriate amount of medium, and the culture plate was incubated at 37°C for 24–96 hours. hawkwind and fire mp3下载