Phospho chk2 antibody
WebBackground: The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA … WebProduct name Anti-Phospho Serine/Threonine Chk1/Chk2 substrate antibody Description Rabbit polyclonal to Phospho Serine/Threonine Chk1/Chk2 substrate Host species Rabbit …
Phospho chk2 antibody
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WebDec 21, 2001 · Phosphorylation and Activation of Chk2 Depend on the Severity of DNA Damage To investigate the phosphorylation and activation of Chk2, we raised polyclonal anti-phospho-Thr 68 antibodies against a Chk2 peptide containing T68P (CETVST (PO 4 )QELYS). The specificity of these affinity-purified antibodies was examined by Western … WebKey features and details Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply APC Rabbit monoclonal [Chk2T68-D12] to Chk2 (phospho T68) Suitable for: Flow Cyt Reacts with: Human Conjugation: APC. Ex: 645nm, Em: 660nm Related conjugates and formulations FITC PE Unconjugated
WebPhospho-Chk2 (Ser19) Antibody detects endogenous levels of Chk2 only when phosphorylated at serine 19. The antibody does not cross-react with Chk2 phosphorylated at other sites. Species Reactivity: Human Source / Purification Web> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M
WebPhospho-CHK2 (Thr68) Antibody (600-401-280) in WB. Western blot using Rocklands Affinity Purified anti-Chk2 pT68 antibody shows detection of a predominant band at ~60 … WebCHK2 (also known as CHEK2, Protein kinase CHK2 isoform a, and checkpoint-like protein) is a serine/ threonine-protein kinase involved in the control of cell cycle checkpoints and …
WebInvitrogen Anti-Phospho-CHK2 (Thr68) Polyclonal, Catalog # PA5-17818. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Flow Cytometry (Flow) and …
WebSep 5, 2000 · ATM-dependent phosphorylation of Chk2 is seen only when cells are damaged by IR. Chk2 is phosphorylated in an ATM-independent manner in response to UV or HU ( 7, … how to take acetaminophen and ibuprofenWebAll lanes : Anti-Chk2 (phospho T387) antibody (ab55319) at 1/500 dilution Lane 1 : Jurkat cell lysate Lane 2 : Jurkat cell lysate with the immunising phosphopeptide. Predicted band … how to take acetylsalicylic acidWebMar 25, 2024 · Flow cytometric analysis of phospho-Chk2 pThr68 on untreated (blue histogram) or UV-treated (green histogram) HeLa cells. Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Chk2 pThr68 polyclonal antibody (Product # PA5-17818) at a 1:800 dilution for 1 hour at room … how to take action against the policeWebJun 30, 2024 · Membranes were incubated in 5% nonfat milk for 45 min at room temperature and then incubated for 18 hours at 4°C with primary antibodies against the following molecules diluted in 5% nonfat milk: p21, p27, phospho-Chk1, Chk1, phospho-Chk2, Chk2, ZEB1, ZEB2, p53, and γH2AX (table S10). how to take act onlineWebThe Phospho-Chk1/2 Antibody Sampler Kit offers an economical means to evaluate the phosphorylation status of Chk1 and Chk2 on multiple residues. The kit contains enough … ready indianaWebHuman Phospho-Chk2 (T68) Antibody Summary Species Reactivity Human Specificity Detects human Chk2 when phosphorylated at T68 in direct ELISAs and Western blots. Source Recombinant Monoclonal Rabbit IgG Clone # 1238H Purification Protein A or G purified from cell culture supernatant Immunogen E.coli -derived recombinant human … how to take acidity out of spaghetti sauceWebPVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CDC2 (Y15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF888), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CDC2 (Y15) at approximately 34 kDa (as indicated). how to take act for free