WebThis is accomplished using the DNA‐ free ™ Kit (Ambion) according to the manufacturer's instructions. RPA probes are generated by PCR amplification of 1 ng of a plasmid DNA template that contains the target sequence. Plasmid templates include the pGL3c plasmid (Promega) for the Photinus luciferase probe and the pCMV‐β plasmid for the lacZ probe. WebApr 13, 2024 · Following RNA extraction, all eukaryotic and prokaryotic samples were treated with DNase using the DNA-free TM Kit (Ambion, Thermofisher Scientific, Harz, Germany). For endothelial RNA, a DNase treatment was carried out according to the manufacturer’s protocol. For bacterial RNA, the protocol was modified.

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WebFeb 2, 2024 · To rid our RNA preparations of any gDNA contamination and prepare pure samples for cDNA synthesis we utilize Ambion's TURBO DNA-free kit. We selected this kit because of its simple, two step … WebOct 27, 2005 · The TURBO DNA-Free Kit is Ambion’s answer to the dilemma of DNase inactivation without damage to RNA samples or affects on downstream reactions. The kit … jeep limited edition

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WebFollowing RNA isolation, remove genomic DNA using Ambion DNA-Free kit following the manufacturer’s instructions. 9. Arbitrarily choose six DNA-free RNA samples and add 1 μL of 100 mg/mL RNase A. These will be used to ensure that there is no contaminating DNA in the samples when the qPCR is run. 10. WebTURBO™ DNA- free™ Kit is similar to the DNA- free Kit but includes TURBO DNase, an engineered hyperactive DNase that exhibits up to 350% greater catalytic efficiency than wild type DNase. The enzyme also has a 6-fold lower K m for DNA, thus enabling effective removal of trace quantities of DNA contamination. jeep locator new