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WebJul 15, 2024 · Then, all cells were carefully layered over 8 ml of cold Biocoll (Biochrom, Germany) with a density of 1.077 g/ml in a 15 ml tube followed by a centrifugation step at 800 × g for 30 min with the ... WebNov 12, 2016 · PBS-Dulbecco’s without Ca 2+ and Mg 2+ (PBS, Biochrom, Germany). 8. Trypsin-EDTA solution (0.25 %, Biochrom, Germany). 9. FACS buffer—PBS-Dulbecco’s solution supplemented with 1 % FCS. 2.6 In Vitro Evaluation of Targeting Efficiency of Folate Receptor Targeted Liposomes by Spectrofluoromety. 1. how to summon a plesiosaur in ark
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